The colorimetric LAMP 2X master mix is an optimized buffer formulation with BST polymerase and it contains Hydroxyl naphthol (HNB) dye is a metal indicator, which enables the visual detection of the Mg2+ concentration and its decrease by pyrophosphat..read more in description section..
The colorimetric LAMP 2X master mix is an optimized buffer formulation with BST polymerase and it contains Hydroxyl naphthol (HNB) dye is a metal indicator, which enables the visual detection of the Mg2+ concentration and its decrease by pyrophosphate precipitation during positive lamp reaction. The addition of HNB to reaction mix, changes colour purple to blue in positive sample during DNA amplification. The mix can be used any LAMP reaction and it requires only heated chamber and sample, which readout of positive amplification analysed by eye in 30-60 min.
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