Product description

Enterocytozoon hepatopenaei (EHP), a recently reported pathogen in the penaeid shrimp, is spreading widely and seriously threatening Penaeus (Litopenaeus) vannamei aquaculture. EHP real time PCR kit contains a specific ready-to-use system for the detection of the EHP by polymerase chain reaction in the real-time PCR system. The master mix contains reagents and enzymes for the specific amplification of the EHP DNA. Fluorescence is emitted and measured by the real time systems´ optical unit. The detection of EHP fragment is performed in fluorimeter channel FAM with the fluorescent quencher BHQ1. Kit included with a ready to use DNA extraction buffer and gill or muscle samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control (1×107copies/ml) contained, allows the determination of the gene load.