Product description

Acute hepatopancreatic necrosis disease (AHPND) means infection with strains of Vibrio parahaemolyticus (Vp AHPND) that contain a ~70-kbp plasmid with genes that encode homologues of the Photorhabdus insect-related (Pir) toxins, PirA and PirB. Although there are reports of the isolation of other Vibrio species from clinical cases of AHPND, only Vp AHPNDhas been demonstrated to cause AHPND. EMS (AHPND) real time PCR kit contains a specific ready-to-use system for the detection of the EMS (AHPND) by polymerase chain reaction in the real-time PCR system. The master mix contains reagents and enzymes for the specific amplification of the EMS () DNA. Fluorescence is emitted and measured by the real time systems´ optical unit. The detection of AHPND fragment is performed in fluorimeter channel FAM with the fluorescent quencher BHQ1. Kit included with a ready to use DNA extraction buffer and gill or muscle samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control (1×107copies/ml) contained, allows the determination of the gene load.