Product description

Like M-MLV reverse transcriptase (RT) and AMV RT, Aura HIV RT synthesizes cDNA from single-stranded RNA template in the presence of primer, Mg 2+, and deoxyribonucleotides. HIV-RT is a recombinant enzyme purified to homogeneity from E. coli. HIV RT is a hetero dimeric protein which is comprised of a 66 kDa and a 51 kDa subunit. HIV RT is More Thermostable than M-MLV RT and AMV RT HIV RT performs as well as M-MLV RT and AMV RT at 37°C and 42°C. However, the enzyme retains a significantly higher fraction of its activity at 50°C than the other RTs. HIV RT converts a much larger proportion of a synthetic RNA transcript to cDNA than M-MLV RT and AMV RT even after the reaction has progressed for 1 hour. Thus, when working with RNA transcripts that are refractory to efficient cDNA synthesis or when the transcript being studied has a high GC content, HIV RT is a superior alternative to M-MLV RT and AMV RT. Aura HIV RT is a high-quality enzyme that has been tested for contaminating endonuclease and exonuclease activity. The enzyme is available in a convenient 250 and 500 U size. A 10X Reaction Buffer is supplied with the enzyme.